Vδ2 TCR Mouse anti-Human, Brilliant Violet 605, Clone: B6, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 743751

Catalog No. BDB743751

Description

The B6 monoclonal antibody specifically recognizes the human variableδ2 subunit (Vδ2) of theγ/δT cell receptor (TCR). Theγ/δTCR is composed of two disulfide linked glycoproteins. The delta chain is approximately 40-60 kDa and is restricted to peripheral blood T cells and thymocytes. The majority of normal peripheral bloodγ/δT cells express a Vγ9[+]/ Vδ2[+] phenotype. The reason for this selection in the T-cell repertoire is not well understood.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.

Specifications

• Antigen: Vδ2 TCR
• Clone: B6
• Conjugate: Brilliant Violet 605
• Gene Alias: TCR Vδ2
• Immunogen: Human TCR Vδ2 Recombinant Protein
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Human
• Isotype: IgG1 κ