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Vγ 3 TCR Hamster anti-Mouse, BUV395, Clone: 536, BD Optibuild™

Syrian Hamster Monoclonal Antibody

Manufacturer:  BD Biosciences 743244

Catalog No. BDB743244

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The 536 monoclonal antibody specifically recognizes Vγ3† T-cell Receptor (TCR)-bearing T lymphocytes, which are the predominantγδTCR-bearing cells in the early fetal thymus and the adult epidermis of euthymic mice. The first T cells to mature in the embryonic thymus express the Vγ3 and Vδ1 TCR chains, and their development is dependent upon IL-7. There is evidence that the Vγ3 TCR-bearing fetal thymocytes are the precursors of the majority of dendritic epidermal T cells (DEC), which may be replenished in the adult by proliferation in situ /rather than by seeding from primary lymphoid organs. Although the Vγ3 TCR is almost exclusively found in the DEC population, it has been shown that the homing of DEC to the epidermis does not require expression of the Vγ3 gene segment. Vγ3 TCR-bearing dermal dendritic cells have also been described. Vγ3 TCR has also been found on a subset of T lymphocytes in the lactating mammary gland and at the site of antigenic challenge in contact-sensitized mice. Plate-bound 536 antibody activates Vγ3 TCR-bearing T cells, and Fab fragments of mAb 536 block in vitro /stimulation of DEC by keratinocytes.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.


Vγ 3 TCR
Aqueous buffered solution containing ≤0.09% sodium azide.
TCR Vγ3, TCR V gamma 3, TCR Vg3
AKR mouse dendritic epidermal cell clone 7-17
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BUV395 under optimal conditions that minimize unconjugated dye and antibody.
Brilliant Ultraviolet 395
Affinity Purified
Syrian Hamster
Syrian Hamster IgG1, κ
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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