Vδ 6.3/2 TCR Hamster anti-Mouse, Brilliant Violet 605, Clone: 8F4H7B7, BD Optibuild™

Catalog No.	Quantity
BDB744474	50 μg

Catalog No. BDB744474 Supplier BD Biosciences Supplier No. 744474

Description

Armenian Hamster Monoclonal Antibody

The 8F4H7B7 antibody reacts with Vδ6.3/2 T-cell Receptor (TCR)-bearing T lymphocytes. Originally defined as a member of the Vδ6 TCR subfamily, it is now proposed that the C57BL-derived Vδ6.3 is an allelic variant of Vδ6.2, found in A/J, AKR, BALB/c, C3H/He, and FVB mice. mAb 8F4H7B7 crossreacts with Vδ6.4 and possibly Vδ6.6 in DBA/2 mice, and it also detects a subset ofγδTCR-bearing cells in CBA/J and C57L mice. It does not recognize Vδ4, Vδ5, Vδ6.1, or Vδ6.5 TCR. A subpopulation of thymocytes expressing Vδ6.3 or Vδ6.4 TCR (in C57BL/6 or DBA/2 mice, respectively) and low levels of CD90.2 (Thy-1.2) shares functional and phenotypic characteristics with NK-T cells. SimilarδV 6.4 TCRexpressing lymphocytes make up significant proportions of theγδT-cell populations in the liver and spleen of DBA/2 mice. Furthermore, T lymphocytes bearing Vδ6.3/2 TCR are found in the skin and intestinal epithelium and may represent a unique T-cell subpopulation with a potential for autoimmune reactivity.

This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser. BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5. These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates. Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.

Specifications

• Antigen: Vδ 6.3/2 TCR
• Classification: Monoclonal
• Clone: 8F4H7B7
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 605
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: V delta 6.3/2 TCR, TCR Vδ 6.3/2, TCR Vδ6.3/2, TCR Vd6.3/2
• Host Species: Armenian Hamster
• Immunogen: 1E6.11.D7, a C57BL/6 mouse Vγ1.1Vδ6.3 TCR-bearing IEL/T-cell hybridoma
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2 κ