Vδ 6.3/2 TCR Hamster anti-Mouse, BUV395, Clone: 8F4H7B7, BD Optibuild™

Catalog No.	Quantity
BDB744478	50 μg

Catalog No. BDB744478 Supplier BD Biosciences Supplier No. 744478

Description

Armenian Hamster Monoclonal Antibody

The 8F4H7B7 antibody reacts with Vδ6.3/2 T-cell Receptor (TCR)-bearing T lymphocytes. Originally defined as a member of the Vδ6 TCR subfamily, it is now proposed that the C57BL-derived Vδ6.3 is an allelic variant of Vδ6.2, found in A/J, AKR, BALB/c, C3H/He, and FVB mice. mAb 8F4H7B7 crossreacts with Vδ6.4 and possibly Vδ6.6 in DBA/2 mice, and it also detects a subset ofγδTCR-bearing cells in CBA/J and C57L mice. It does not recognize Vδ4, Vδ5, Vδ6.1, or Vδ6.5 TCR. A subpopulation of thymocytes expressing Vδ6.3 or Vδ6.4 TCR (in C57BL/6 or DBA/2 mice, respectively) and low levels of CD90.2 (Thy-1.2) shares functional and phenotypic characteristics with NK-T cells. SimilarδV 6.4 TCRexpressing lymphocytes make up significant proportions of theγδT-cell populations in the liver and spleen of DBA/2 mice. Furthermore, T lymphocytes bearing Vδ6.3/2 TCR are found in the skin and intestinal epithelium and may represent a unique T-cell subpopulation with a potential for autoimmune reactivity.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: Vδ 6.3/2 TCR
• Classification: Monoclonal
• Clone: 8F4H7B7
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Ultraviolet 395
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: V delta 6.3/2 TCR, TCR Vδ 6.3/2, TCR Vδ6.3/2, TCR Vd6.3/2
• Host Species: Armenian Hamster
• Immunogen: 1E6.11.D7, a C57BL/6 mouse Vγ1.1Vδ6.3 TCR-bearing IEL/T-cell hybridoma
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2 κ