Vβ 6 T-Cell Receptor Rat anti-Mouse, Brilliant Violet 510, Clone: RR4-7, BD Optibuild™

Catalog No.	Quantity
BDB744591	50 μg

Catalog No. BDB744591 Supplier BD Biosciences Supplier No. 744591

Description

Rat Monoclonal Antibody

The RR4-7 antibody specifically reacts with the V β 6 T-Cell Receptor (TCR) of mice having the a ( e.g., C57BR, C57L, SJL) and b ( e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the Tcrb gene complex. The Tcrb-V6 gene locus is deleted in mice having the c ( e.g., RIII) haplotype. V β 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by Mtv-7 ( Mls-1[a], Mls[a] ) endogenous provirus ( e.g., AKR, CBA/J, C58, DBA/2, NZB), or Mtv -43 endogenous provirus ( e.g., MA/MyJ). Exogenous MMTV-SW, as well as endogenous Mtv -44-encoded superantigen ( e.g., NZW), also causes incomplete elimination of V β 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates V β 6 TCR-bearing T cells, soluble RR4-7 mAb blocks in vitro proliferation and cytolytic activities of V β 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of V β 6 TCR-bearing T cells.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.

Specifications

• Antigen: Vβ 6 T-Cell Receptor
• Classification: Monoclonal
• Clone: RR4-7
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 510
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: TCR V beta 6, TCR Vβ6
• Host Species: Rat
• Immunogen: C57BL/6 Mouse Helper T-Cell Clone OI11
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b λ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov