Vβ 6 T-Cell Receptor Rat anti-Mouse, Brilliant Violet 786, Clone: RR4-7, BD Optibuild™

Catalog No.	Quantity
BDB744595	50 μg

Catalog No. BDB744595 Supplier BD Biosciences Supplier No. 744595

Description

Rat Monoclonal Antibody

The RR4-7 antibody specifically reacts with the V β 6 T-Cell Receptor (TCR) of mice having the a ( e.g., C57BR, C57L, SJL) and b ( e.g., A, BALB/c, CBA/Ca, C3H/He, C57BL, DBA/1) haplotypes of the Tcrb gene complex. The Tcrb-V6 gene locus is deleted in mice having the c ( e.g., RIII) haplotype. V β 6 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by Mtv-7 ( Mls-1[a], Mls[a] ) endogenous provirus ( e.g., AKR, CBA/J, C58, DBA/2, NZB), or Mtv -43 endogenous provirus ( e.g., MA/MyJ). Exogenous MMTV-SW, as well as endogenous Mtv -44-encoded superantigen ( e.g., NZW), also causes incomplete elimination of V β 6 TCR-expressing T cells. Plate-bound RR4-7 antibody activates V β 6 TCR-bearing T cells, soluble RR4-7 mAb blocks in vitro proliferation and cytolytic activities of V β 6 TCR-bearing T-cell clones, and injection of the antibody results in in vivo depletion of V β 6 TCR-bearing T cells.

The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm. BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter). BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm. BV786 offers a bright choice for the 6th detector off the violet laser.

Specifications

• Antigen: Vβ 6 T-Cell Receptor
• Classification: Monoclonal
• Clone: RR4-7
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 786
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: TCR V beta 6, TCR Vβ6
• Host Species: Rat
• Immunogen: C57BL/6 Mouse Helper T-Cell Clone OI11
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2b λ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov