Vβ 8.1, 8.2 TCR Mouse anti-Mouse, Brilliant Violet 421, Clone: MR5-2, BD Optibuild™

Catalog No.	Quantity
BDB744329	50 μg

Catalog No. BDB744329 Supplier BD Biosciences Supplier No. 744329

Description

Mouse Monoclonal Antibody

The MR5-2 antibody reacts with the V β 8.1 and V β 8.2 T-cell Receptors (TCR), but not the V β 8.3 TCR, of mice having the b haplotype ( e.g ., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a ( e.g ., C57BR, C57L, SJL, SWR) or c ( e.g., RIII) haplotype. V β 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by the Mtv-7 (Mls-1a, Mlsa), provirus ( e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of V β 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 (e.g., MA/MyJ), Mtv-44 (e.g., NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of V β 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, V β 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing V β 8 TCR and selectively eliminates those T cells in vivo . Plate-bound MR5-2 antibody activates V β 8.1 or 8.2 TCR-bearing T lymphocytes.

The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: Vβ 8.1, 8.2 TCR
• Classification: Monoclonal
• Clone: MR5-2
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 421
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: TCR V beta 8.1, TCR V beta 8.2
• Host Species: Mouse
• Immunogen: C57BL/6 mouse helper T-cell clone OI6
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov