Vβ 8.1, 8.2 TCR Mouse anti-Mouse, Brilliant Violet 711, Clone: MR5-2, BD Optibuild™

Catalog No.	Quantity
BDB744333	50 μg

Catalog No. BDB744333 Supplier BD Biosciences Supplier No. 744333

Description

Mouse Monoclonal Antibody

The MR5-2 antibody reacts with the V β 8.1 and V β 8.2 T-cell Receptors (TCR), but not the V β 8.3 TCR, of mice having the b haplotype ( e.g ., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a ( e.g ., C57BR, C57L, SJL, SWR) or c ( e.g., RIII) haplotype. V β 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen encoded by the Mtv-7 (Mls-1a, Mlsa), provirus ( e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of V β 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 (e.g., MA/MyJ), Mtv-44 (e.g., NZW), and/or exogenous MMTV-SW superantigens also cause incomplete elimination of V β 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, V β 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing V β 8 TCR and selectively eliminates those T cells in vivo . Plate-bound MR5-2 antibody activates V β 8.1 or 8.2 TCR-bearing T lymphocytes.

The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes. This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm. BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. However, the spillover can be corrected through compensation as with any other dye combination. BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm. This dye offers a very bright choice for the violet laser. Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors. BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

Specifications

• Antigen: Vβ 8.1, 8.2 TCR
• Classification: Monoclonal
• Clone: MR5-2
• Concentration: 0.2mg/mL
• Conjugate: Brilliant Violet 711
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: TCR V beta 8.1, TCR V beta 8.2
• Host Species: Mouse
• Immunogen: C57BL/6 mouse helper T-cell clone OI6
• Purification Method: Affinity Purified
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Mouse
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov