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Vβ 8 T-Cell Receptor Mouse anti-Mouse, Brilliant Violet 510, Clone: F23.1, BD Optibuild™

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 742377

Catalog No. BDB742377


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Description

Description

The F23.1 antibody specifically reacts with the V β 8.1, V β 8.2, and V β 8.3 T-cell receptors (TCR) of mice having the b haplotype ( e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a ( e.g., C57BR, C57L, SJL, SWR) or c ( e.g., RIII) haplotype. V β 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by Mtv-7 (Mls-1a, Mlsa) provirus ( e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of V β 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of V β 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, V β 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing V β 8 TCR and selectively eliminates those T cells in vivo . Soluble and plate-bound F23.1 antibody activates V β 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by V β 8 TCR-bearing cytotoxic T lymphocytes, and in vivo treatment of neonatal mice can arrest intrathymic maturation of V β 8 TCR-bearing T cells.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.
Specifications

Specifications

Vβ 8 T-Cell Receptor
0.2mg/mL
Aqueous buffered solution containing ≤0.09% sodium azide.
TCR V beta 8, TCR V beta 8.1/8.2/8.3
BALB.B Mouse Lymph-Node and Spleen T Cells
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with BD Horizon BV510 under optimal conditions that minimize unconjugated dye and antibody.
RUO
Primary
Murine
F23.1
Brilliant Violet 510
Affinity Purified
Murine
Mouse C57L IgG2a, κ
50μg
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Monoclonal
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