Vβ 8 T-Cell Receptor Mouse anti-Mouse, BUV395, Clone: F23.1, BD Optibuild™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 742382

Catalog No. BDB742382

Description

The F23.1 antibody specifically reacts with the V β 8.1, V β 8.2, and V β 8.3 T-cell receptors (TCR) of mice having the b haplotype ( e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex. The Tcrb-V8 subfamily gene loci are deleted in mice having the a ( e.g., C57BR, C57L, SJL, SWR) or c ( e.g., RIII) haplotype. V β 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by Mtv-7 (Mls-1a, Mlsa) provirus ( e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of V β 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E. Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of V β 8.1 TCR-bearing T cells. In addition to expression on conventional T lymphocytes, V β 8.2 is the predominant β chain of the TCR on NK-T cells. Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing V β 8 TCR and selectively eliminates those T cells in vivo . Soluble and plate-bound F23.1 antibody activates V β 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by V β 8 TCR-bearing cytotoxic T lymphocytes, and in vivo treatment of neonatal mice can arrest intrathymic maturation of V β 8 TCR-bearing T cells.

The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry. With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter. BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences. With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter. This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.

Specifications

• Antigen: Vβ 8 T-Cell Receptor
• Clone: F23.1
• Conjugate: Brilliant Ultraviolet 395
• Gene Alias: TCR V beta 8, TCR V beta 8.1/8.2/8.3
• Immunogen: BALB.B Mouse Lymph-Node and Spleen T Cells
• Quantity: 50 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov