Invitrogen™ VMA2 Monoclonal Antibody (13D11B2)

Catalog No.	Quantity
A6427	250 μg

Catalog No. A6427 Supplier Invitrogen™ Supplier No. A6427

Description

Mouse Monoclonal Antibody

This clone recognizes the 60kDa subunit 1 (B-subunit, the product of the VMA2 or VAT2 gene) of S. cerevisiae. By Western blot, this can be used to identify organelles during subcellular fractionation of yeast membranes.

Vacuolar-type H+-ATPase (V-ATPase) is a multisubunit enzyme responsible for acidification of eukaryotic intracellular organelles. V-ATPases pump protons against an electrochemical gradient, while F-ATPases reverse the process, thereby synthesizing ATP. A peripheral V1 domain, which is responsible for ATP hydrolysis, and a integral V0 domain, which is responsible for proton translocation, compose V-ATPase. Nine subunits (A-H) make up the V1 domain and five subunits (a, d, c, c' and c ) make up the V0 domain. Like F-ATPase, V-ATPase most likely operates through a rotary mechanism. The V-ATPase V1 B subunit exists as two isoforms. In the inner ear, the V-ATPase B1 isoform functions in proton secretion and is required to maintain proper endolymph pH and normal auditory function. The gene encoding the human V-ATPase B1 isoform maps to chromosome 2cen-q13. Mutations in this gene cause distal renal tubular acidosis associated with sensorineural deafness. The V-ATPase B2 isoform is expressed in kidney and is the only B isoform expressed in osteoclasts.

Specifications

• Antigen: VMA2
• Applications: ELISA, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: 13D11B2
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: HEPES buffered saline with 0.02% sodium azide
• Gene: VMA2
• Gene Alias: ATP6B1; ATP6V1B1; ATPase H+ transporting V1 subunit B1; ATPase, H transporting, lysosomal V1 subunit B1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta 56/58 kDa, isoform 1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1; ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1 (Renal tubular acidosis with deafness); ATPase, H+ transporting, lysosomal V1 subunit B1; ATPase, H+ transporting, V1 subunit B, isoform 1; AW208839; D630003L15; D630030L16Rik; D630039P21Rik; endomembrane proton pump 58 kDa subunit; H(+)-transporting two-sector ATPase, 58kD subunit; H+-ATPase beta 1 subunit; lysosomal 56/58kDa; RTA1B; vacuolar H+-ATPase; vacuolar proton pump 3; vacuolar proton pump subunit B 1; vacuolar proton pump, subunit 3; VATB; V-ATPase B1; V-ATPase B1 subunit; V-ATPase subunit B 1; VMA2; VPP3; Vpp-3; V-type proton ATPase subunit B, kidney isoform
• Gene Symbols: VMA2
• Host Species: Mouse
• Immunogen: Purified S. cerevisiae vacuole membranes.
• Purification Method: Purified
• Quantity: 250 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 852424
• Target Species: Yeast
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1