V5 Epitope Tag Rabbit anti-Tag, DyLight™ 800, Polyclonal, Rockland™

Rabbit Polyclonal Antibody

Supplier:  Rockland Immunochemicals 600445378

Catalog No. 50-199-2535

Description

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This affinity purified antibody is directed against the V5 epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the V5 epitope tag (Gly-Lys-Pro-Ile-Pro-Asn-Pro-Leu-Leu-Gly-Leu-Asp-Ser-Thr) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Specifications

• Antigen: V5 Epitope Tag
• Classification: Polyclonal
• Conjugate: DyLight 800
• Gene Alias: SPV5gp2; V5; V5 Epitope Tag
• Quantity: 100 μg
• Primary or Secondary: Primary
• Product Type: Antibody
• Target Molecule: Tag

• Applications: ELISA, Immunofluorescence, Western Blot
• Concentration: 1 mg/mL
• Formulation: 0.02M potassium phosphate with 0.15M NaCl, 10 mg/mL BSA and 0.01% sodium azide; pH 7.2
• Host Species: Rabbit
• Regulatory Status: RUO
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Lyophilized