Invitrogen™ VAMP4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA1768

Catalog No. PA1768

Description

PA1-768 detects VAMP-4 from human and mouse samples. PA1-768 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~18 kDa protein representing VAMP-4 from PC3 cell extract. Immunofluorescent staining of VAMP-4 in CV-1 cells using PA1-768 results in a staining pattern consistent with VAMP-4 staining. Immunofluorescence staining with this antibody requires saponin permeabilization. The PA1-768 antigen is recombinant rat VAMP-4.

The vesicle associated membrane proteins (VAMP) or synaptobrevins are calcium binding proteins specific to eukaryotes. VAMPs, along with synaptosomal associated protein of 25 kDa (SNAP-25) and syntaxin, form the core complex of soluble NSF attachment protein receptor (SNARE) proteins that interact with the soluble proteins N-ethylmaleimide-sensitive factor (NSF) and alpha-SNAP. These membrane associated proteins play a key role in the regulation of vesicle membrane fusion with the plasma membrane. The Clostridium tetani neurotoxin is a metalloprotease with specificity for VAMP. In Alzheimer's disease, VAMP levels of all isoforms appear to be significantly lowered.

Specifications

• Antigen: VAMP4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: VAMP4
• Gene Alias: D1Ertd147e; vamp; VAMP24; VAMP4; VAMP-4; vesicle associated membrane protein 4; vesicle-associated membrane protein 4
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 364033, 53330, 8674
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 1 mg/mL
• Formulation: PBS with 1 mg/mL BSA and 0.05% sodium azide
• Gene Accession No.: O70480, O75379
• Gene Symbols: VAMP4
• Immunogen: Recombinant rat VAMP-4 protein.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov