MilliporeSigma™ VANGL2, Rat, Unlabeled, Clone: 2G4,

Catalog No.	Quantity	Conjugate
MABN750	200 μL	Unconjugated

Catalog No. MABN750 Supplier MilliporeSigma™ Supplier No. MABN750

Description

Rat Monoclonal Antibody

Sorting nexin-17 (UniProt Q15036) is encoded by the SNX17 (also known as KIAA0064) gene (Gene ID 9784) in human. The sorting nexins (SNXs) constitute a family of adaptor proteins involved in proteins trafficking through the endosomal pathway. Originally identified as a binding partner of the cell adhesion protein P-selectin, sorting nexin-17 (SNX17) has since been shown to mediate internalization and recycling of multiple cell surface proteins including P-selectin, amyloid precursor protein (APP), integrin β1, low density lipoprotein receptor (LDLR), LDLR related protein (Lrp1), ApoER2, and FEEL1. All SNX proteins contain a conserved phox homology (PX) domain that mediates phosphoinositides binding in a SNX type-specific manner, as well as various scaffolding modules including SH3, BAR, and PDZ domains. SNX17 PX domain (a.a. 1-109) displays specificity for phosphatidylinositol 3-monophosphate, which is enriched at the cytoplasmic face of the early endosome. SNX17 also contains a 4.1 protein/ezrin/radixin/moesin (FERM) domain (a.a. 115-432) comprised of an ubiquitin/Ras association (RA) subdomain known as the F1 lobe (a.a. 113-205), an F2 lobe (a.a. 206-269) with a helical structure similar to acyl-CoA binding protein, and an F3 lobe (a.a. 270-388) similar to the phosphotyrosine binding (PTB)/pleckstrin homology domain. The FERM domain mediates interaction with Asn-Pro-Xaa-Tyr/Phe (NPX(Y/F) motif-containing proteins for internalization as well as interaction with adaptor proteins-containing PTB (or FERM) domains to trigger signaling events.

Specifications

• Antigen: VANGL2
• Applications: Immunofluorescence, Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: 2G4
• Concentration: Please refer to lot specific datasheet.
• Conjugate: Unconjugated
• Formulation: Purified rat IgG2a in buffer containing 0.1M Tris-Glycine (pH 7.4), 150mM NaCl with 0.05% Sodium Azide.
• Gene: Vangl2, Lpp1, Ltap, Stb1, Stbm
• Gene Accession No.: Q91ZD4
• Host Species: Rat
• Immunogen: GST-tagged recombinant VANGL2 N-terminal fragment 100% conserved among human, mouse, and rat species.
• Purification Method: Protein G Purified
• Quantity: 200 μL
• Regulatory Status: RUO
• Research Discipline: Neuroscience
• Primary or Secondary: Primary
• Gene ID (Entrez): NP_277044
• Test Specificity: Clone 2G4 reacted specifically with VANGL2, but not VANGL1. Clone 2G4 detected shRNA-mediated VANGL2 downregulation in SK-BR-7 human breast carcinoma cells (Belotti, E., et al. (2012). PLoS One. 7(9):e46213).
• Target Species: Human
• Content And Storage: Stable for 1 year at 2°-8°C from date of receipt.
• Form: Purified
• Isotype: IgG2a κ

For Research Use Only. Not for use in diagnostic procedures.