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PeproTech VCAM-1 Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No. 500P30050UG
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500P30050UG 50 μg
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Rabbit Polyclonal Antibody

AA Seq. of rec. protein: FKIETTPESR YLAQIGDSVS LTCSTTGCES PFFSWRTQID SPLNGKVTNE GTTSTLTMNP VSFGNEHSYL CTATCESRKL EKGIQVEIYSF PKDPEIHLSG PLEAGKPITV KCSVADVYPF DRLEIDLLKG DHLMKSQEFL EDADRKSLET KSLEVTFTPV IEDIGKVLVC RAKLHIDEMD SVPTVRQAVK ELQVYISPKN TVISVNPSTK LQEGGSVTMT CSSEGLPAPE IFWSKKLDNG NLQHLSGNAT LTLIAMRMED SGIYVCEGVN LIGKNRKEVE LIVQEKPFTV EISPGPRIAA QIGDSVMLTC SVMGCESPSF SWRTQIDSPL SGKVRSEGTN STLTLSPVS FENEHSYLCT VTCGHKKLEK GIQVELYSFPR DPEIEMSGGLV NGSSVTVSCK VPSVYPLDRLE IELLKGETILE NIEFLEDTDM KSLENKSLEMT FIPTIEDTGKA LVCQAKLHID DMEFEPKQRQ STQTLYVNVA PRDTTVLVSP SSILEEGSSV NMTCLSQGFP APKILWSRQL PNGELQPLSE NATLTLISTK MEDSGVYLCE GINQAGRSRK EVELIIQVTP KDIKLTAFPS ESVKEGDTVI ISCTCGNVPE TWIILKKKAE TGDTVLKSID GAYTIRKAQL KDAGVYECES KNKVGSQLRS LTLDVQGREN NKDYFSP. Sandwich ELISA: To detect Hu VCAM-1 by sandwich ELISA (using 100 uL/well antibody solution) a conc. of 0.5-2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Hu VCAM-1 (500-P300Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of rec. Hu VCAM-1. WB: To detect hVCAM-1 by WB analysis this antibody can be used at a conc. of 0.1-0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for rec. hVCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
TRUSTED_SUSTAINABILITY

Specifications

Antigen VCAM-1
Applications ELISA, Western Blot
Classification Polyclonal
Concentration 0.1-1.0 mg/mL
Conjugate Unconjugated
Formulation PBS with no preservative
Gene VCAM1
Gene Accession No. P19320
Gene Alias adhesion molecule VCAM-1; CD106; CD106 antigen; DKFZp779G2333; INCAM100; INCAM-100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; vascular cell adhesion molecule; vascular cell adhesion molecule 1; vascular cell adhesion molecule-1; vascular cell adhesion protein 1; V-CAM 1; Vcam1; Vcam-1; VCAM1B
Gene Symbols VCAM1
Host Species Rabbit
Immunogen HEK293 cells-derived Recombinant Human VCAM-1.
Purification Method Antigen affinity chromatography
Quantity 50 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7412
Target Species Human
Content And Storage -20°C
Product Type Antibody
Form Lyophilized
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