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VCP Monoclonal Antibody (Hs-14), Invitrogen™
Mouse Monoclonal Antibody
Supplier: Thermo Scientific MA110383
SDSDocumentsProduct Certifications Promotions
For ICC staining, membrane permeabilization with acetone is essential. Purity is >95% by SDS-PAGE.VCP (valosin-containing protein), is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm, this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
|valosin-containing protein, p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, Transitional endoplasmic reticulum ATPase|
|selective precipitation, Size-exclusion chromatography|
|4° C, do not freeze|
|Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot|
|Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.|
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