VCP Monoclonal Antibody (Hs-14), Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Thermo Scientific MA110383

Catalog No. PIMA110383

Description

For ICC staining, membrane permeabilization with acetone is essential. Purity is >95% by SDS-PAGE.

VCP (valosin-containing protein), is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm, this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.

Specifications

• Antigen: VCP
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Gene: VCP
• Gene Alias: valosin-containing protein, p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, 15S Mg(2+)-ATPase p97 subunit, TER ATPase, Transitional endoplasmic reticulum ATPase
• Host Species: Mouse
• Purification Method: selective precipitation, Size-exclusion chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 269523, 7415
• Content And Storage: 4° C, do not freeze
• Form: Liquid

• Applications: Flow Cytometry, Immunocytochemistry, Immunofluorescence, Western Blot
• Clone: Hs-14
• Conjugate: Unconjugated
• Gene Accession No.: P55072, Q01853
• Gene Symbols: VCP
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgM