Invitrogen™ VCP Monoclonal Antibody (Hs-14)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA110383

Catalog No. PIMA110383

Description

This antibody reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein. For ICC staining, membrane permeabilization with acetone is essential.

The protein encoded by this gene is a member of a family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. This protein, as a structural protein, is associated with clathrin, and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation.

Specifications

• Antigen: VCP
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: TBS with 15mM sodium azide; pH 8.0
• Gene Accession No.: P55072, Q01853
• Gene Symbols: VCP
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgM

• Applications: Flow Cytometry, Western Blot, Immunocytochemistry
• Clone: Hs-14
• Conjugate: Unconjugated
• Gene: VCP
• Gene Alias: 15S Mg(2+)-ATPase p97 subunit; 3110001E05; AAA ATPase p97; ALS14; CDC48; cell division cycle gene CDC48; CMT2Y; epididymis luminal protein 220; epididymis secretory protein Li 70; HEL-220; HEL-S-70; homolog of yeast cdc48; IBMPFD; IBMPFD1; Inv protein; or HEL-S-70; p97; p97 atpase; p97/VCP; Protein CDC48; si:ch211-113n10.2; TER ATPase; TERA; transitional endoplasmic reticulum ATPase; valosin containing protein; valosin-containing protein; Vcp; wu:fd16d05; wu:fj63d11; yeast Cdc48p homolog
• Host Species: Mouse
• Purification Method: Sequential Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 269523, 7415
• Content And Storage: 4°C, do not freeze
• Form: Liquid