Invitrogen™ VDAC Monoclonal Antibody (N152B/23), PerCP

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA545668

Catalog No. PIMA545668

Description

Mouse: 98% identity (279/283 amino acids identical). Rat: 98% identity (279/283 amino acids identical) >60% identity with VDAC2 and VDAC3. 1 μg/mL of MA5-45668 was sufficient for detection of VDAC1 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 30kDa. Does not cross-react with VDAC2 or VDAC3 (based on KO validation results). This antibody was formerly sold as clone S152B-23.

Voltage-dependent anion channel (VDAC) proteins are abundant, pore-forming proteins belonging to the eukaryotic mitochondrial porins. It was discovered in the mitochondrial outer membrane. It also is expressed in the plasma membrane. At least three different VDAC genes have been identified in vertebrates. VDAC proteins are known to play an essential role in cellular metabolism and in the early stages of apoptosis. For example, VDAC constitutes a major pathway by which metabolites such as ADP/ATP, succinate and citrate are exchanged between the cytosol and mitochondria. And VDAC1 in the plasma membrane establishes a novel level of apoptosis regulation putatively via its redox activity.

Specifications

• Antigen: VDAC
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: 2.48mM MES, 95.46mM phosphate with 2mM EDTA and no preservative; pH 7.4
• Gene Accession No.: P21796, Q60932, Q9Z2L0
• Gene Symbols: VDAC1
• Immunogen: Fusion protein amino acids 1-283 (full-length) of human VDAC1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG2a

• Applications: Immunohistochemistry, Western Blot, Immunocytochemistry
• Clone: N152B/23
• Conjugate: PerCP
• Gene: VDAC1
• Gene Alias: AL033343; BR1-VDAC; Brain-derived voltage-dependent anion channel 1; hVDAC1; MGC111064; Mitochondrial outer membrane protein porin 1; Mitochondrial Porin; mVDAC1; mVDAC5; N2441; OMP2; Outer mitochondrial membrane protein porin 1; plasmalemmal porin; POR1; PORIN; Porin 31HL; porin 31HM; porin POR1; PORIN-31-HL; rVDAC1; VD; VDAC; VDAC1; VDAC-1; Vdac5; VDAC-5; voltage dependent anion channel 1; voltage-dependent anion channel 1; voltage-dependent anion-selective channel protein 1; Voltage-dependent anion-selective channel protein 5; YNL055C; YNL2441C
• Host Species: Mouse
• Purification Method: Protein G
• Regulatory Status: RUO
• Gene ID (Entrez): 22333, 7416, 83529
• Content And Storage: 4°C
• Form: Liquid