Invitrogen™ VDR Polyclonal Antibody

Catalog No.	Quantity
PA1711	100 μL

Catalog No. PA1711 Supplier Invitrogen™ Supplier No. PA1711

Description

Rabbit Polyclonal Antibody

PA1-711 detects vitamin D receptor (VDR) from human, mouse and rat tissues. PA1-711 has been successfully used in Western blot, immunoprecipitation and gel shift procedures. By Western blot, this antibody detects an ~53 kDa protein representing VDR in COS-7 cells transfected with the human gene. PA1-711 supershifts DNA fragments that contain VDR response elements (e.g., rat osteocalcin and mouse osteopontin upstream elements). PA1-711 immunizing peptide corresponds to amino acid residues 395-413 from human VDR. Recombinant human VDR (Cat. # RP-710) and human RXR beta (catalog # RP-812) proteins can be purchased separately for control experiments in Western blot, immunoprecipitation and gel shift procedures.

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.

Specifications

• Antigen: VDR
• Applications: Gel Shift, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: Conc. Not Determined
• Conjugate: Unconjugated
• Formulation: Whole serum with 0.05% sodium azide
• Gene: VDR
• Gene Accession No.: O42392, P11473, P13053, P48281
• Gene Alias: 1,25-dihydroxyvitamin D3 receptor; HGNC:12679; I79_025795; Nr1i1; Nuclear receptor subfamily 1 group I member 1; PPP1R163; protein phosphatase 1, regulatory subunit 163; vdr; vdr.S; vdr-A; vitamin D (1,25- dihydroxyvitamin D3) receptor; vitamin D (1,25- dihydroxyvitamin D3) receptor S homeolog; vitamin D (1,25-dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D receptor; vitamin D receptor protein; vitamin D receptor S homeolog; Vitamin D3 receptor; XELAEV_18015993mg; xVDR
• Gene Symbols: VDR
• Host Species: Rabbit
• Immunogen: Synthetic Peptide: E(395) E H S K Q Y R C L S F Q P E C S M K(413).
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 22337, 24873, 395988, 7421
• Target Species: Human, Mouse, Rat, Chicken
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG