Invitrogen™ VDR Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595259

Catalog No. PIPA595259

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: Human Placenta Tissue, Rat Kidney Tissue, Rat Liver Tissue, Rat Pancreas Tissue, HELA whole cell. IHC: mouse intestine tissue, rat intestine tissue, human intestinal cancer tissue. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. The 1,25-dihydroxy-vitamin D3 receptor (VDR) belongs to the superfamily of steroid hormone receptors which includes estrogen, progesterone, glucocorticoid, androgen, and thyroid hormone receptors. Vitamin D modulates calcium and phosphorus homeostasis, bone remodeling, cell growth regulation, and differentiation. Studies have found VDR in the intestine, bone, kidney, epidermis, and cells of the endocrine immune system. Repression of T-cell proliferation and interleukin-2 (IL-2) gene expression, for example, occurs when VDR binds within the IL-2 enhancer. Formation of VDR/retinoic X receptor (RXR) heterodimers in the presence of intracellular 1,25(OH)2D3 has been shown to interfere with assembly of nuclear factor of activated T-cells (NFATp)/Fos/Jun/DNA complex and subsequent IL-2 gene transcription. The corresponding gene for the vitamin D receptor is NR1I1.

Specifications

• Antigen: VDR
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: VDR
• Gene Alias: 1,25-dihydroxyvitamin D3 receptor; HGNC:12679; I79_025795; Nr1i1; Nuclear receptor subfamily 1 group I member 1; PPP1R163; protein phosphatase 1, regulatory subunit 163; vdr; vdr.S; vdr-A; vitamin D (1,25- dihydroxyvitamin D3) receptor; vitamin D (1,25- dihydroxyvitamin D3) receptor S homeolog; vitamin D (1,25-dihydroxyvitamin D3) receptor; vitamin D nuclear receptor variant 1; vitamin D receptor; vitamin D receptor protein; vitamin D receptor S homeolog; Vitamin D3 receptor; XELAEV_18015993mg; xVDR
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22337, 24873, 7421
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene Accession No.: P11473, P13053, P48281
• Gene Symbols: VDR
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human VDR (377-402aa HLLYAKMIQKLADLRSLNEEHSKQYR).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG