VEGF-121Rabbit anti-Human, Polyclonal, Rockland™

Catalog No.	Quantity
50-199-1400	100 μg

Catalog No. 50-199-1400 Supplier Rockland Immunochemicals Supplier No. 209401C23

Description

Rabbit Polyclonal Antibody

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for human VEGFA-121 protein. A BLAST analysis was used to suggest cross-reactivity with VEGFA-121 from human sources based on 100% homology with the immunizing sequence. Based on high to 100% homology, there is a chance of cross-reactivity to VEGFA-121 from a wide variety of animals. Cross-reactivity with VEGFA-121 from other sources has not been determined.

VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

Specifications

• Antigen: VEGF-121
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and no preservative; pH 7.2
• Gene: VEGFA
• Gene Accession No.: P15692
• Gene Alias: angiogenic factor; BOS_21465; etID309976.16; eVEGF120; eVEGF164; human VEGF121 homolog; MGC70609; MVCD1; ovine vascular endothelial growth factor; RP1-261G23.1; VAS; vascular endothelial growth factor; vascular endothelial growth factor 121 isoform; vascular endothelial growth factor 164; vascular endothelial growth factor 165b; vascular endothelial growth factor 182 isoform; vascular endothelial growth factor 188; vascular endothelial growth factor 188 isoform; Vascular endothelial growth factor A; vascular endothelial growth factor A121; vascular endothelial growth factor A165; vascular endothelial growth factor Aa; Vascular endothelial growth factor A-A; vascular endothelial growth factor isoform 165; Vascular permeability factor; Vasculotropin; vegf; VEGF1; VEGF164; VEGF182; VEGF188; vegfa; VEGF-A; vegfaa; VEGF-A-A; VEGFMGC70609; VPF; VPF ant; wu:fj82c06
• Gene Symbols: VEGFA
• Host Species: Rabbit
• Immunogen: This IgG fraction antibody was prepared from rabbit antiserum after repeated immunizations with mature length recombinant human VEGFA-121 protein produced in E.coli.
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7422
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Lyophilized