PeproTech VEGFB Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Catalog No.	Quantity
50-271-8553	500 μg
500P267BT25	25 μg
500P267BT50	50 μg

Catalog No. 50-271-8553 Supplier PeproTech Supplier No. 500P267BT500UG

Description

Rabbit Polyclonal Antibody

AA Sequence of recombinant protein: PVSQPDAPGH QRKVVSWIDV YTRATCQPRE VVVPLTVELM GTVAKQLVPS CVTVQRCGGC CPDDGLECVP TGQHQVRMQI LMIRYPSSQL GEMSLEEHSQ CECRPKKKDS AVKPDSPRPL CPRCTQHHQR PDPRTCRCRC RRRSFLRCQG RGLELNPDTC RCRKLRR. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human VEGF-B. Anti-Human VEGF-B-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human VEGF-B by sandwich ELISA (using 100 μL/well) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human VEGF-B (500-P267) as a capture antibody, allows the detection of at least 2000-4000 pg/mL of Recombinant Human VEGF-B. Western Blot: To detect Human VEGF-B by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible development reagents the detection limit for Recombinant Human VEGF-B is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P267BT-1MG will be provided as 2 x 500 μg

Vascular endothelial growth factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGFs stimulate endothelial cells, induce angiogenesis, promote cell migration, increase vascular permeability, and inhibit apoptosis. VEGFB has structural similarities to VEGF and PIGF and is frequently co-expressed with VEGF. There are two alternatively spliced isoforms of VEGFB: VEGFB 167 and VEGFB 186. VEGFB 167, a highly basic heparin-binding protein, remains with the cell or extracellular matrix whereas, VEGFB 186 is readily secreted. VEGFB stimulates endothelial cell proliferation. VEGFB binds to the tyrosine kinase receptor VEGFR1 (flt1) and does not bind to VEGFR2. Vascular Endothelial Growth Factor B is widely expressed but is most abundant in heart, skeletal muscle, and pancreas. It has been suggested that VEGFB expression in human primary breast cancers is associated with lymph node metastasis. The genes that encode VEGFB have been mapped to chromosome 11q13.

Specifications

• Antigen: VEGFB
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.1-1.0 mg/mL
• Conjugate: Biotin
• Formulation: PBS with no preservative
• Gene: VEGFB
• Gene Accession No.: P49765
• Gene Alias: flt-1/VEGFR1 ligand; vascular endothelial growth factor B; vascular endothelial growth factor B167 precursor; vascular endothelial growth factor B186 precursor; vascular endothelial growth factor-B; Vegfb; VEGF-B; VEGFL; VEGF-related factor; Vrf
• Gene Symbols: VEGFB
• Host Species: Rabbit
• Immunogen: E.coli-derived Recombinant Human VEGF-B.
• Purification Method: Antigen affinity chromatography
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7423
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG