Thermo Scientific™ Verso cDNA Synthesis Kit

Description

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool.

The Thermo Scientific Verso cDNA Synthesis Kit provides robust transcription of RNA to create a complete cDNA pool. The Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increasing transcription efficiency, and the cDNA synthesis buffer has been optimized to achieve a full and diverse cDNA pool.

Features of the Verso cDNA Synthesis Kit include:
• Highly sensitive with a broad dynamic range—can reverse transcribe RNA from an input range of 1pg to 1μg
• Efficient RT reaction allows for 75% shorter protocol times (down to 30 minutes)
• Unique priming strategy for increased efficiency and broad template flexibility
• High affinity RT enzyme effectively transcribes through difficult RNA sequences
• Wide working temperature range (42–57°C) for success with GC-rich and other difficult templates
• Included RT enhancer prevents genomic DNA carryover, eliminating the need for separate DNase I treatment
• RNase inhibitor included
• Can be paired with any PCR Kit for complete 2-Step RT-(q)PCR

Applications
• 2-step RT-(q)PCR
• Single stranded cDNA synthesis

Specifications

• Content And Storage:

  • RT Enzyme mix with RNase Inhibitor
  • 5X RT Buffer
  • Random Hexamers
  • Anchored Oligo (dT)
  • 20 mM dNTPs
  • RT Enhancer
  
  Store at –20°C.
  
  

• Format: Kit
• Reaction Speed: 30 min.
• Technique: Reverse Transcription
• Optimal Reaction Temperature: 42°C
• Quantity: 40 reactions
• Reverse Transcriptase: Verso
• Ribonuclease H Activity: Yes
• Shipping Condition: Wet or Dry Ice
• For Use With (Application): Real Time PCR (qPCR), RT-PCR
• Final Product Type: First-Strand cDNA
• No. of Reactions: 40 Reactions
• Reaction Format: Separate components
• Reagent Type: Reverse Transcription
• Size (Final Product): Up to 11 kb
• Starting Material: RNA

For Research Use Only. Not for use in diagnostic procedures.