MilliporeSigma™ Vesicular Glutamate Transporter 3 (VGLUT3), Guinea Pig, Unlabeled, Polyclonal,

Guinea Pig Polyclonal Antibody

Supplier:  MilliporeSigma™ AB5421I

Catalog No. AB5421IMI

Description

Low-density lipoprotein receptor (UniProt P01130; also known as LDL receptor) is encoded by the LDLR (also known as FH, FHC) gene (Gene ID 3949) in human. Low-density lipoprotein (LDL) receptor recognizes the apoprotein B100 embedded in the outer phospholipid layer of cholesterol-rich LDL particles and mediates their remval by endocytosis via clathrin-coated pits. LDL receptor also recognizes the apoE protein found in chylomicron remnants and VLDL remnants (IDL). LDLR gene mutations are known causes of familial hypercholesterolaemia (FH). LDL receptor is initially produced with a signal peptide sequence (a.a. 1-21), the removal of which yields the mature single transmembrane (a.a. 789-810) protein wiht a C-terminal cytoplasmic tail (a.a. 811-860) and a large N-terminal extracellular region (a.a. 22-788) that contains seven class A (a.a. 25-313) and six class B (a.a. 397-658) LDLR repeats, as well as three EGF-like domains (a.a. 314-353, 354-393, 663-712). LDL-A repeats mediate LDL binding, repeats 2-7 are required for ApoB binding, while only repeat 5 is required for ApoE binding. The class B (LDL-B) repeats region adopts a beta-propeller conformation and is believed to be responsible for the pH-dependent release of LDL in the endosome. The cytoplasmic tail contains a signal sequence important for localizing the receptors to clathrin-coated pits and for triggering receptor-mediated endocytosis upon LDL binding.

Specifications

• Antigen: Vesicular Glutamate Transporter 3 (VGLUT3)
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Slc17a8, Vglut3
• Host Species: Guinea Pig
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This polyclonal guinea pig antiserum was raised against a VGluT3 C-terminal cytoplasmic domain sequence conserved between rat and murine species and shares 96% homology with the corresponding human sequence.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Guinea pig polyclonal antibody serum with 0.05% Sodium Azide.
• Gene Accession No.: Q7TSF2
• Immunogen: KLH-conjugated linear peptide corresponding to a C-terminal cytoplasmic domain sequence of rat vesicular glutamate transporter 3 (VGluT3).
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_714947
• Target Species: Human
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.