MilliporeSigma™ VGluT2, Guinea Pig, Unlabeled, Polyclonal

Guinea Pig Polyclonal Antibody

Supplier:  MilliporeSigma™ AB2251I

Catalog No. AB2251IMI

Description

AB2251-I is a Guinea pig polyclonal antibody that detects VGluT2 and is tested for use in Immunohistochemistry (Paraffin), and Western Blotting.  Vesicular glutamate transporter 2 (UniProt Q9JI12; also known as Differentiation-associated BNPI, Differentiation-associated Na(+)-dependent inorganic phosphate cotransporte, Solute carrier family 17 member 6, VGluT2) is encoded by the Slc17a6 (also known as Dnpi, Vglut2) gene (Gene ID 84487) in rat species. Vesicular glutamate transporters (VGluTs) are proton-dependent carriers responsible for the upload of the major excitatory neurotransmitter glutamate into synaptic vesicles within presynaptic terminals to allow later regulated release at the synaptic cleft. Studies conducted in mice show that VGluT1 and VGluT2 are essential for vital functions, while Vglut3-knockout mice have a normal life expectancy despite being deaf and developing anxiety and discrete basal locomotor phenotypes. VgluT1- and VGluT2-expressing neurons represent the bulk of canonical glutamatergic neurons in the brain, with VgluT1 representing the major subtype and accounting for approximately 80% of total vesicular transport of glutamate in the brain. In contrast, VgluT3 is found in discrete population of non-glutamatergic neurons using other transmitters than glutamate, such as cholinergic interneurons in the dorsal and ventral striatum, subpopulations of GABAergic basket cells in the cortex and hippocampus, and serotoninergic neurons, where it is involved in more subtle modulation of local transmission. Rat VGluT2 is a 12-transmembrane (a.a. 72-92, 126-146, 149-169, 178-198, 217-237, 245-265, 311-331, 350-370, 387-407, 410-430, 444-464, 478-498) protein with 7 cytoplasmic and 6 vacuolar domains, having both its N- and C-terminal tails (a.a. 1-71 and 499-582) exposed at the cytoplasmic side.

Specifications

• Antigen: VGluT2
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Slc17a6, Dnpi, Vglut2
• Host Species: Guinea Pig
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This polyclonal antibody targets VGluT2 C-terminal end sequence.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• Applications: Western Blot
• Concentration: Please refer to lot specific datasheet.
• Formulation: Guinea pig polyclonal antibody serum with 0.05% Sodium Azide.
• Gene Accession No.: Q9JI12
• Immunogen: KLH-conjugated linear peptide corresponding to the C-terminal sequence of rat VGluT2.
• Quantity: 100 μL
• Research Discipline: Neuroscience
• Gene ID (Entrez): NP_445879
• Target Species: Mouse
• Form: Serum

For Research Use Only. Not for use in diagnostic procedures.