Invitrogen™ Villin Monoclonal Antibody (SP145)

Rabbit Monoclonal Antibody

Supplier:  Invitrogen™ MA516408

Catalog No. PIMA516408

Description

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon, small intestine or kidney tissue.

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

Specifications

• Antigen: Villin
• Classification: Monoclonal
• Concentration: 0.108 mg/mL
• Formulation: PBS with 1% BSA and 0.1% sodium azide; pH 7.6
• Gene Accession No.: P09327, Q62468
• Gene Symbols: VIL1
• Immunogen: Synthetic peptide derived from the C-terminus of human villin protein.
• Quantity: 500 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: SP145
• Conjugate: Unconjugated
• Gene: VIL1
• Gene Alias: D2S1471; OTTHUMP00000207159; Vil; Vil1; villin 1; villin-1
• Host Species: Rabbit
• Purification Method: Protein A/G
• Regulatory Status: RUO
• Gene ID (Entrez): 22349, 7429
• Content And Storage: 4°C, do not freeze
• Form: Liquid