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Vimentin Antibody (VM1170), Novus Biologicals™
SDP

Catalog No. p-5539926 Shop All R&D Systems Products
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NBP24483300 0.02 mg
NBP24483301 0.1 mg
NBP24483302 0.2 mg
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Catalog No. NBP24483300 Supplier Novus Biologicals Supplier No. NBP2448330.02MG
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Description

Mouse Monoclonal Antibody

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Vimentin Monoclonal specifically detects Vimentin in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

Antigen Vimentin
Applications Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
Classification Monoclonal
Clone VM1170
Concentration 0.2mg/mL
Conjugate Unconjugated
Dilution Western Blot 0.25 - 0.5 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml, Immunofluorescence 1 - 2 ug/ml
Formulation 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
Gene Accession No. P08670
Gene Alias FLJ36605, vimentin
Gene Symbols VIM
Host Species Mouse
Immunogen Recombinant full-length human vimentin protein
Purification Method Protein A or G purified
Quantity 0.02 mg
Regulatory Status RUO
Research Discipline Cancer, Cellular Markers, Cytoskeleton Markers, Growth and Development, Hypoxia, Neuronal Cell Markers, Neuronal Stem Cell Markers, Neuroscience, Signal Transduction, Stem Cell Markers, Stem Cells
Primary or Secondary Primary
Gene ID (Entrez) 7431
Test Specificity This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Target Species Human, Mouse (Negative), Rat (Negative)
Content And Storage Store at 4C.
Form Purified
Isotype IgG1
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