Vimentin/gp100, MultiVision Ab Cocktail is one marker in a comprehensive antibody portfolio that addresses all major disease states, ensuring the ideal tests are always available.
The antibody to vimentin (clone SP20) reacts with the main intermediate filament protein in mesenchymal cells and is therefore of value in the differential diagnosis of undifferentiated neoplasms. Melanoma antibody gp100, clone HBM45, specifically recognizes a 100 kDa protein in melanocytes and melanomas. Intradermal nevi, normal adult melanocytes and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial or mesenchymal origin. Vimentin-Melanoma double staining contributes to the diagnosis of melanoma in skin since abundant co-localization is observed in HMB45 positive melanoma cells. Because malignant melanomas may show a mixture of HMB45 positive and negative cells, single-vimentin positive melanoma cells are also observed. Vimentin and gp100 antibodies are provided at optimal concentrations in an easy-to-use format for visualizing melanoma positive cells in red and blue, respectively and/ or co-localization marked by a purple/brownish mixed-color. The use of Rabbit Monoclonal Vimentin allows for superior staining, in both sensitivity and specificity, permiting the use of higher titers compared to mouse monoclonal antibodies. For detection of the primary antibodies a ready-to-use MultiVision polymer detection system consisting of anti-mouse/horseradish peroxidase (HRP) + anti-rabbit/alkaline phosphatase (AP) (Mfr. No. TL-012-MHRA) is available. This cocktail is specially formulated from MultiVision polymers that provide increased sensitivity, time-savings, and detection simplicity. The MultiVision AP and HRP polymers are innovative, patented technology. The smaller amino acid polymer subunits minimize conflicts in binding the target protein resulting in more consistent staining and better signal amplification. Ultimately, this gives the user higher sensitivity and antibody efficiency as well as better signal-to-noise ratios. This system is biotin-free, which prevents background staining found with traditional biotin-based methods. MultiVision polymer detection system is a simple and robust one-step polymer detection system for double staining of rabbit antibody to Vimentin and mouse antibody to Melanoma gp100 (clone HMB45) in blue and red, respectively.The MultiVision polymer detection system yields high quality double staining in less than 2 hours on formalin-fixed and paraffin-embedded tissue sections. The kit allows observation of two single-stained cells in blue (Vimentin) and red (Melanoma gp100) and/or co-localization marked by a purple/brownish mixed-color.
Host Species: Vimentin — Rabbit; gp100 — Mouse
Clone: Vimentin — SP20; gp100 — HMB45
Isotype: Vimentin — Rabbit monoclonal IgG; gp100 — Mouse IgG1/κ
Species Reactivity: Human. Others not known.
Epitope: Vimentin — Not determined; gp100 — Not determined
Immunogen: Vimentin — recombinant protein encoding human Vimentin; gp100 — Extract of pigmented melanoma metastases from lymph nodes
Molecular Weight: Vimentin — 57-60kDa; gp100—100kDa
Positive Control: Melanoma
Cellular Localization: Vimentin — Cytoplasmic; gp100 — Cytoplasmic This product is intended for qualitative immunohistochemistry with normal and neoplastic formalin-fixed, paraffin-embedded tissue sections, to be viewed by light microscopy.
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