Invitrogen™ Vimentin Monoclonal Antibody (RV202)

Catalog No.	Quantity
OMA106001	100 μL

Catalog No. OMA106001 Supplier Invitrogen™ Supplier No. OMA106001

Description

Mouse Monoclonal Antibody

OMA1-06001 detects vimentin from mouse, rat, hamster, chicken, goat, canine, monkey, zebrafish, porcine and human tissues and cells. OMA1-06001 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry (frozen) procedures. By Western blot, this antibody detects a ~57 kDa protein representing vimentin from human mesenchymal cells. OMA1-06001 antigen is cytoskeletal vimentin extract of calf lens. Store at 4°C or at -20°C if preferred. Avoid freeze-thaw cycles.

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Unlike other IFP proteins, vimentin is expressed, along with desmin, during the early stages of cellular development. During the development process, vimentin is exchanged for new, tissue-specific IFPs. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Vimentin phosphorylation by a protein kinase causes the breakdown of intermediate filaments and activation of an ATP and myosin light chain dependent contractile event. This results in cytoskeletal changes that facilitate the interaction of the lipid droplets within mitochondria, and subsequent transport of cholesterol to the organelles leading to an increase in steroid synthesis.

Specifications

• Antigen: Vimentin
• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: RV202
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 0.09% sodium azide
• Gene: Vim
• Gene Accession No.: P02543, P08670, P09654, P20152, P31000, P48616, P48670
• Gene Alias: cb28; class-III intermediate microfilament; CTRCT30; epididymis luminal protein 113; FLJ36605; Hel113; intermediate filament; intermediate filament protein; RP11-124N14.1; similar to vimentin; VIM; VIME; Vimentin; vimentin C-terminus
• Gene Symbols: Vim
• Host Species: Mouse
• Immunogen: Cytoskeletal vimentin extract of calf lens.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 100522394, 100689099, 102182515, 140599, 22352, 280955, 420519, 477991, 7431, 81818
• Target Species: Bovine, Canine, Chicken, Goat, Hamster, Human, Mouse, Monkey, Pig, Rat, Frog, Frog
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1