Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More
  1. Home
  2. Products
  3. Antibodies
  4. Primary Antibodies
  5. All Primary Antibodies
  6. Invitrogen™ Vinculin Monoclonal Antibody (7F9), Alexa Fluor™ 488, eBioscience™, Invitrogen™ 

Invitrogen™ Vinculin Monoclonal Antibody (7F9), Alexa Fluor™ 488, eBioscience™, Invitrogen™
GREENER_CHOICE

Catalog No. 501124646
Encompass_Preferred
Change view
Click to view available options
Quantity:
100 μg
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
50-112-4646 100 μg
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 50-112-4646 Supplier Invitrogen™ Supplier No. 53977782
Add to Cart
Edge
Add to Cart

Description

Mouse Monoclonal Antibody

Description: The monoclonal antibody 7F9 (VIIF9) recognizes human, mouse, rat, and avian vinculin and its alternatively spliced isoform, metavinculin. Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions. Vinculin is involved in the anchoring of F-actin to the membrane and the regulation of E-cadherin expression. Vinculin binds to talin, paxillin, and alpha-actinin. Disregulation of vinculin alters cell adhesion, migration, and growth, which promotes cancer invasion and metastasis. Applications Reported: This 7F9 antibody has been reported for use in immunohistochemical staining of frozen tissue sections, immunohistochemical staining of formalin-fixed paraffin embedded tissue sections, microscopy, and immunocytochemistry. Applications Tested: This 7F9 antibody has been tested by immunocytochemistry of formaldehyde-fixed and permeabilized cells and can be used at less than or equal to 20 μg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Vinculin
Applications Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunocytochemistry
Classification Monoclonal
Clone 7F9
Concentration 0.5 mg/mL
Conjugate Alexa Fluor 488
Formulation PBS with 0.09% sodium azide; pH 7.2
Gene VCL
Gene Accession No. P18206, P85972, Q64727
Gene Alias 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
Gene Symbols VCL
Host Species Mouse
Purification Method Affinity chromatography
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 22330, 305679, 7414
Target Species Human, Mouse, Rat
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG1 κ
Show More Show Less

For Research Use Only.

Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.