Invitrogen™ Vinculin Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA585279

Catalog No. PIPA585279

Description

Keep as concentrated solution. Predicted reactivity: Mouse (98%), Rat (97%), Pig (99%), Chicken (93%), Bovine (99%). Positive Control: PC-12, Rat2, Jurkat, Raji, NCI-H929, Mouse muscle. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Vinculin (VCL) is a cytoskeletal protein associated with cell-cell and cell-extracellular matrix adherens-type junctions. It functions as one of several interacting proteins involved in anchoring F-actin to the membrane. It has been shown that a sequence of molecular interactions might be involved in the transmembrane assembly of adhesion plaques. In the assembly of adhesion plaques, the beta subunit of integrin binds to talin. Talin binds to vinculin that interacts with alpha-actinin and possibly with itself. Since alpha-actinin binds to and cross-links actin filaments, vinculin represents a key element in the transmembrane linkage of the extracellular matrix to the cytoplasmic microfilament system. Mutations in the gene can result in cardiomyopathy dilated 1W or familial hypertrophic 15.

Specifications

• Antigen: Vinculin
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: VCL
• Gene Alias: 9430097D22; AA571387; AI462105; AW545629; CMD1W; CMH15; epididymis luminal protein 114; HEL114; metavinculin; meta-vinculin; metavinculin {ECO:0000250; MV; MVCL; RP11-178G16.3; UniProtKB:P18206}; unnamed protein product; Vcl; vcl {ECO:0000250; VIN; vinc; vinc 1; VINC1; Vinculin; vinculin {ECO:0000250
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22330, 305679, 7414
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.11 mg/mL
• Formulation: PBS with 1% BSA, 20% glycerol and 0.025% ProClin 300; pH 7
• Gene Accession No.: P18206, P85972, Q64727
• Gene Symbols: VCL
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Vinculin. The exact sequence is proprietary.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG