Gibco™ Viral Production Medium, AGT™

Description

Gibco Viral Production Medium, AGT, is the dry-format version of CTS Viral Production Medium, a chemically defined, serum-free, protein-free, animal origin-free, phenol red-free medium.

• Gibco Viral Production Medium, AGT is the dry-format version of CTS Viral Production Medium, a chemically defined, serum-free, protein-free, animal origin-free, phenol red-free medium
• Medium is part of the CTS AAV-MAX Helper-Free AAV Production System for high-titer adeno-associated virus (AAV) production
• The CTS AAV-MAX Helper-Free AAV Production System is a scalable, high-titer platform for suspension AAV production with research-grade and GMP versions to enable seamless transition from research to commercial manufacturing
• Other components of the CTS AAV-MAX system include CTS Viral Production Cells 2.0, CTS AAV-MAX Transfection Kit, and CTS Viral-Plex Complexation Buffer.

Features of Viral Production Medium, AGT, include:

• Supports growth and transfection of CTS Viral Production Cells 2.0 from shake flask to bioreactor scales
• Helps enable production of high AAV titers, greater than 5 x 10¹⁰ vg/mL (unpurified)
• Simplifies transfer to manufacturing scale due to Advanced Granulation Technology (AGT) enabling auto-pH and simplified dissolution
• Formulated with GlutaMAX Supplement, no further supplementation required
• Manufactured in conformity with cGMP for medical devices, 21 CFR, Part 820, and certified to ISO 13485. Drug Master File and Regulatory Support File available upon request.

Advanced Granulation Technology

• Advanced Granulation Technology (AGT) helps simplify medium preparation at large scale with fast rehydration
• A just-add water protocol, and low dust generation, creates less pressure build-up during sterile filtration
• For more information on AGT media preparation at large scale, see Hydration and scale-up of AGT medium in HyPerforma and imPULSE Single-Use Mixers

Specifications

• Cell Line: Viral Production Cells 2.0
• Cell Type: HEK 293 cells
• Concentration: 25.6 g/L
• Form: Powder (AGT)
• Product Type: AAV Production Medium
• Sterility: Non-sterile
• With Additives: Glucose, GlutaMAX
• Without Additives: No Phenol Red, No Antibiotics
• For Use With (Application): AAV Production
• Product Line: AAV-MAX
• Quantity: 100 L
• pH: 6.9 to 7.5
• Reconstituted Volume: 100 L
• Content And Storage: 100 L Ropack Bucket, store at 2°C to 8°C protected from light
• Culture Type: Suspension Cell Culture
• Expression System: Mammalian