Invitrogen™ VISTA Monoclonal Antibody (MIH64), Brilliant Violet™ 650, eBioscience™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
41-610-8380	Invitrogen™ 416108380	25 μg
41-610-8382	Invitrogen™ 416108382	100 μg

Description

Description<br>This MIH64 monoclonal antibody recognizes mouse VISTA, also known as B7-H5 or PD-H1. This MIH64 antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells.<br><br>Applications Tested<br>This MIH64 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1.0 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.<br><br>Blocking Buffers<br>When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401</a>) or Brilliant Stain Buffer (Product # 00-4409-75</a>) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.<br><br>Light sensitivity<br>This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.<br><br>Fixation<br> Samples can be stored in IC Fixation Buffer (Product # 00-8222...

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

Specifications

• Antigen: VISTA
• Concentration: 0.2 mg/mL
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 650
• Host Species: Rat
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9D659
• Gene ID (Entrez): 74048
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: MIH64
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Mouse
• Gene: VSIR
• Gene Alias: 4632428N05Rik; B7H5; B7-H5; C10orf54; C1H10orf54; C28H10orf54; C4H10orf54; chromosome 10 open reading frame 54; DD1alpha; Death Domain1alpha; Dies1; differentiation of ESCs 1; GI24; PD-1H; PDCD1 homolog; PD-H1; platelet receptor Gi24; PP2135; RIKEN cDNA 4632428N05 gene; SISP1; sisp-1; Stress-induced secreted protein-1; UNQ730/PRO1412; V-domain Ig suppressor of T cell activation; VISTA; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; VSIR; V-type immunoglobulin domain-containing suppressor of T-cell activation
• Gene Symbols: VSIR
• Product Type: Antibody