VISTA Rat anti-Mouse, Brilliant Violet 421, Clone: MIH64, BD

Rat Monoclonal Antibody

Supplier:  BD Biosciences 566271

Catalog No. BDB566271

Description

The MIH64 monoclonal antibody specifically binds to V-domain Ig suppressor of T cell activation (VISTA) which is also known as, Differentiation of ESCs 1 (Dies1). VISTA is a type I transmembrane glycoprotein that belongs to the Ig superfamily. VISTA has homology to the B7 family ligand, PD-L1 (CD274), and is likewise known as PD-1H. VISTA is expressed on naοve and activated T cells, natural killer (NK) cells, macrophages, dendritic cells, and neutrophils. VISTA functions as an inhibitory ligand. It can serve as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. It is also involved in regulating stem cell differentiation.

The antibody was conjugated to BD Horizon BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates. BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences. Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates. Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

Specifications

• Antigen: VISTA
• Clone: MIH64
• Conjugate: Brilliant Violet 421
• Gene Alias: Vista, PD-1H, Dies1
• Immunogen: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
• Host Species: Rat
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Mouse
• Isotype: IgG2a κ