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QuidelOrtho VITROS™ Immunodiagnostic Products Anti-SARS-CoV-2 IgG Controls
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Controls
Description
The VITROS™ Immunodiagnostic Products Anti-SARS-CoV-2 IgG test is performed using the VITROS™ Anti-SARS-CoV-2 IgG Reagent Pack and the VITROS™ Anti-SARS-CoV-2 IgG Calibrator on the VITROS™ ECi/ECiQ/3600 Immunodiagnostic Systems and the VITROS™ 5600/XT 7600 Integrated Systems.
An immunometric technique is used; this involves a two stage reaction.
- In the first stage antibodies to SARS-CoV-2 present in the sample bind with SARS-CoV-2 spike protein coated on wells. Unbound sample is removed by washing.
- In the second stage horseradish peroxidase (HRP)-labeled murine monoclonal anti-human IgG antibodies are added in the conjugate reagent. The conjugate binds specifically to the antibody portion of the antigen-antibody complex. If complexes are not present, the unbound conjugate is removed by the subsequent wash step. The bound HRP conjugate is measured by a luminescent reaction.
- A reagent containing luminogenic substrates (a luminol derivative and a peracid salt) and an electron transfer agent is added to the wells. The HRP in the bound conjugate catalyzes the oxidation of the luminol derivative, producing light. The electron transfer agent (a substituted acetanilide) increases the level of light produced and prolongs its emission. The light signals are read by the system. The amount of HRP conjugate bound is indicative of the amount of SARS-CoV-2 IgG antibody present.
Control Contents:
- 3 sets of VITROS™ Anti‐SARS-CoV-2 IgG Controls
Materials Required but Not Provided:
- VITROS™ Immunodiagnostic Products Signal Reagent
- VITROS™ Immunodiagnostic Products Universal Wash Reagent
- VITROS™ Immunodiagnostic Products Anti-SARS-CoV-2 IgG Reagent Pack (Part No. 6199919)
- VITROS™ Immunodiagnostic Products Anti-SARS-CoV-2 IgG Calibrator (Part No. 6199920)
- VITROS™ Immunodiagnostic Products Reagent Pack Storage Box (optional) with desiccant
Specifications
Specifications
Content And Storage | 2°C to 8°C |
For Use With (Equipment) | VITROS ECi/ECiQ/3600 Immunodiagnostic Systems and the VITROS 5600/XT 7600 Integrated Systems |
Sample Type | Serum |
For Use With (Application) | Assesses immune response by qualitatively measuring IgG antibodies to SARS-CoV-2 |
Packaging | 3 Sets |
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