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Molecular Probes™ Vybrant™ DyeCycle™ Green Stain
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Quantity:
400 μL, 200 reactions
Description
Vybrant™ DyeCycle™ Green Stain is a cell-permeable DNA dye that can be used for cell cycle analysis on a 488 nm spectral line of the argon-ion laser, which is commonly used on all flow cytometers.
Precise—accurate cell cycle analysis in living cells
Safe—low cytotoxicity for cell sorting and additional live cell experiments
Simple, robust staining protocol
View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.
Precise
Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Green Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).
Low Cytotoxicity
Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Green Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
Simple, Robust Staining Protocol
For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2μL of Vybrant™ DyeCycle™ Green Stain. Final stain concentration is 10μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on a flow cytometer using 488 nm excitation and green emission.
View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.
Precise
Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Green Stain is an ideal tool for DNA content analysis in living cells since the stains are cell permeable and, after binding double-stranded DNA, emit a fluorescent signal that is proportional to the DNA mass (see figure).
Low Cytotoxicity
Unlike stains that require high concentrations or have chemical structures that are toxic to cells, the Vybrant™ DyeCycle™ Green Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.
Simple, Robust Staining Protocol
For cell analysis, simply prepare flow cytometry tubes containing 1 mL of cells suspended in complete media at a concentration of 1 × 106 cells/mL. To each tube add 2μL of Vybrant™ DyeCycle™ Green Stain. Final stain concentration is 10μM. Incubate at 37°C for 30 minutes, protected from light. Analyze samples on a flow cytometer using 488 nm excitation and green emission.
Specifications
Specifications
| Concentration | 5 mM |
| Content And Storage | Contains 1 vial of Vybrant™ DyeCycle™ green stain (5 mM in DMSO). Store at ≤-20°C, desiccate and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Flow Cytometer |
| Product Type | Stain |
| Dye Type | Vybrant™ DyeCycle™ Green |
| Emission | 488 |
| Form | Solution |
| Format | Tube(s) |
| Product Line | DyeCycle |
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For Research Use Only. Not for use in diagnostic procedures.
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