Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Molecular Probes™ Vybrant™ DyeCycle™ Ruby Stain, 100 reactions

Catalog No. 501137619
Encompass_Preferred
Click to view available options
Quantity:
100 assays

Vybrant™ DyeCycle™ Ruby Stain

Vybrant™ DyeCycle™ Ruby Stain is a cell permeable DNA dye that can be used for cell cycle analysis on a 488 nm or 635 nm red diode laser.

  • Precise—accurate cell cycle analysis in living cells

  • Safe—low cytotoxicity for cell sorting and additional live cell experiments

  • Minimal compensation—easier multiplexing

  • Simple, robust staining protocol

    View a selection guide for all products related to cell cycle analysis of fixed and live cells in flow cytometry.

    Precise

    Successful cell cycle analysis requires a dye that is DNA selective and can stain cells in a homogeneous pattern minimizing fluorescence variability. The Vybrant™ DyeCycle™ Ruby Stain is an ideal tool for DNA content analysis in living cells since the stain is cell permeable and, after binding double-stranded DNA, emits a fluorescent signal that is proportional to the DNA mass (see figure).

    Low Cytotoxicity

    Unlike stains that require high concentrations or have chemical structures that are toxic to cells, Vybrant™ DyeCycle™ Ruby Stain exhibits relatively low cytotoxicity, allowing the possibility of sorting based on the phase of the cell cycle.

    Minimal Compensation

    Well-suited for the popular red 633 nm laser line, the Vybrant™ DyeCycle™ Ruby Stain/DNA complex has fluorescence excitation and emission maxima of 638/686 nm, respectively (see figure). The spectral characteristics allow use of the red laser for cell cycle analysis and frees up other channels for additional parameters.

    The red excitation and narrow emission of Vybrant™ DyeCycle™ Ruby Stain make it ideal for multiplexing due to the limited spectral overlap with other common dyes (Alexa Fluor™ 488, FITC, and RPE) and fluorescent proteins (Green Fluorescent Protein (GFP)).

    Simple, Robust Staining Protocol

    For cell analysis, simply prepare flow cytometry tubes containing 0.5 mL of cells suspended in complete media at a concentration of 5 × 105 cells/mL. To each tube, add 1μL of Vybrant™ DyeCycle™ Ruby Stain and mix well. Final stain concentration is 5μM. Incubate at 37°C for 15–30 minutes, protected from light. Analyze without washing cells on a flow cytometer using 488 nm or 633/5 nm excitation and >670 nm emission.
  • TRUSTED_SUSTAINABILITY

    Specifications

    Content And Storage Contains 1 vial of Vybrant™ DyeCycle™ ruby stain (2.5 mM in DMSO). Store at ≤-20°C, desiccated and protected from light.
    Detection Method Fluorescence
    For Use With (Equipment) Flow Cytometer
    Product Type Stain
    Dye Type Vybrant™ DyeCycle™ Ruby
    Emission near IR
    Form Solution
    Format Tube(s)
    Product Line DyeCycle, Molecular Probes
    Quantity 100 assays
    Shipping Condition Room Temperature
    Solubility DMSO (Dimethylsulfoxide)
    Sub Cellular Localization Nucleic Acids
    Show More Show Less

    For Research Use Only. Not for use in diagnostic procedures.

    Product Content Correction

    The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

    Product Title

    By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

    Your feedback has been submitted: Thank you for helping us improve our website.