WASF1 (phospho Y125) Rabbit anti-Human, Mouse, Polyclonal , Abnova™

Rabbit polyclonal antibody raised against synthetic phosphopeptide of human WASF1.

Supplier:  Abnova Corporation PAB29590

Catalog No. 89-956-831

Description

The protein encoded by this gene, a member of the Wiskott-Aldrich syndrome protein (WASP)-family, plays a critical role downstream of Rac, a Rho-family small GTPase, in regulating the actin cytoskeleton required for membrane ruffling. It has been shown to associate with an actin nucleation core Arp2/3 complex while enhancing actin polymerization in vitro. Wiskott-Aldrich syndrome is a disease of the immune system, likely due to defects in regulation of actin cytoskeleton. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Specifications

• Antigen: WASF1 (phospho Y125)
• Classification: Polyclonal
• Description: Rabbit polyclonal antibody raised against synthetic phosphopeptide of human WASF1.
• Formulation: In PBS (without Mg²⁺ and Ca²⁺), 150 mM NaCl, pH 7.4 (50% glycerol, 0.02% sodium azide)
• Gene Symbols: WASF1
• Immunogen: A synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding Y125 of human WASF1.
• Quantity: 100 μL
• Primary or Secondary: Primary
• Test Specificity: WASF1 (phospho Y125) polyclonal antibody detects endogenous levels of human WASF1 only when phosphorylated at tyrosine 125.
• Content And Storage: Store at -20°C.
  Aliquot to avoid repeated freezing and thawing.
• Isotype: IgG

• Applications: Immunohistochemistry (PFA fixed), Western Blot
• Conjugate: Unconjugated
• Dilution: Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (1:50-1:100) Western Blot (1:500-1:3000) The optimal working dilution should be determined by the end user.
• Gene Alias: FLJ31482/KIAA0269/SCAR1/WAVE/WAVE1
• Host Species: Rabbit
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 8936
• Target Species: Human, Mouse
• Form: Liquid