Invitrogen™ WASH1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA551731

Catalog No. PIPA551731

Description

Immunogen sequence: GAPREVVDPS GGWATLLESI RQAGGIGKAK LRSMKERKLE KKKQKEQEQV RATSQGGHLM SDLFNKLVMR HKGISGKGPG AGEGPGGAFA RVSDSIPPLP PPQQPQVDED EDDWES Highest antigen sequence identity to the following orthologs: Mouse - 87%, Rat - 89%.

WASHC1 (WASH1) is a protein coding gene with endocytosis as a related pathway. Acts as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. Its assembly in the WASH core complex seems to inhibit its NPF activity and via WASHC2 is required for its membrane targeting. Involved in endocytic trafficking of EGF. Involved in transferrin receptor recycling. Regulates the trafficking of endosomal alpha5beta1 integrin to the plasma membrane and involved in invasive cell migration. In T-cells involved in endosome-to-membrane recycling of receptors including T-cell receptor (TCR), CD28 and ITGAL; proposed to be implicated in T cell proliferation and effector function. In dendritic cells involved in endosome-to-membrane recycling of major histocompatibility complex (MHC) class II probably involving retromer and subsequently allowing antigen sampling, loading and presentation during T-cell activation. Involved in Arp2/3 complex-dependent actin assembly driving Salmonella typhimurium invasion independent of ruffling. Involved in the exocytosis of MMP14 leading to matrix remodeling during invasive migration and implicating late endosome-to-plasma membrane tubular connections and cooperation with the exocyst complex. Involved in negative regulation of autophagy independently from its role in endosomal sorting by inhibiting BECN1 ubiquitination to inactivate PIK3C3/Vps34 activity.

Specifications

• Antigen: WASH1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WASHC1
• Gene Alias: CXYorf1-like protein on chromosome 9; FAM39E; family with sequence similarity 39, member E; FLJ00075 protein; Protein FAM39E; WAS protein family homolog 1; WASH; WASH complex subunit 1; WASH1; WASHC1
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100287171
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Form: Liquid

• Applications: Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 0.1 mg/mL
• Formulation: PBS with 40% glycerol and 0.02% sodium azide; pH 7.2
• Gene Accession No.: A8K0Z3
• Gene Symbols: WASHC1
• Immunogen: Recombinant protein corresponding to Human WASH1. Recombinant protein control fragment (Product #RP-92149).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG