Invitrogen™ WEE1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595636

Catalog No. PIPA595636

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human placenta tissue, human A431 whole cell, human U2OS whole cell. IHC: human mammary cancer tissue. ICC/IF: U20S cell. Flow: SiHa cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

WEE1, which is classified as a Ser/Thr kinase by sequence analysis, has been shown to inactivate multiple CDKs by tyrosine phosphorylation. Wee1 protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.

Specifications

• Antigen: WEE1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WEE1
• Gene Alias: CTRCT30; DKFZp686I18166; epididymis luminal protein 113; fa20h03; FLJ16446; HEL113; VIM; Vimentin; wee 1 homolog; WEE 1 homolog 1 (S. pombe); Wee1; WEE1 G2 checkpoint kinase; WEE1 homolog; wee1 protein; WEE1 tyrosine kinase; WEE1+ homolog; Wee1A; wee1A kinase; WEE1hu; wee1-like protein kinase; wu:fa20h03; zgc:136514
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22390, 308937, 7465
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and 0.05mg sodium azide
• Gene Accession No.: P30291, P47810, Q63802
• Gene Symbols: WEE1
• Immunogen: A synthetic peptide corresponding to a sequence of human WEE1 (ELNAEKFKNSLLQKELKKAQMAKAAAEERALFTDR).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG