Wnt1Rabbit anti-Bovine, Canine, Human, Marsupial, Mouse, Rat, Rhesus Monkey, Polyclonal, Rockland™

Catalog No.	Quantity
50-199-1925	100 μg

Catalog No. 50-199-1925 Supplier Rockland Immunochemicals Supplier No. 600401A37

Description

Rabbit Polyclonal Antibody

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This antibody reacts with human and mouse Wnt1 protein. A BLAST analysis was used to suggest cross-reactivity with Wnt1 from mouse, human, rat, bovine, dog, macaque, and opossum based on a 100% homology with the immunizing sequence. Partial cross-reactivity is expected against chicken Wnt1 based on a 91% sequence homology. Cross-reactivity with Wnt1 from other sources has not been determined.

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

Specifications

• Antigen: Wnt1
• Applications: ELISA, Immunoprecipitation, Western Blot
• Classification: Polyclonal
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Formulation: 0.02M potassium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7.2
• Gene: WNT1
• Gene Accession No.: P04426, P04628
• Gene Alias: BMND16; INT1; Int-1; OI15; Proto-oncogene Int-1; Proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; Proto-oncogene Wnt-1; sw; swaying; wingless-related MMTV integration site 1; Wingless-type MMTV integration site 1 homolog; Wingless-type MMTV integration site 1, homolog; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1); Wnt family member 1; WNT1; Wnt-1
• Gene Symbols: WNT1
• Host Species: Rabbit
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 22408, 24881, 486560, 540662, 709009, 7471
• Target Species: Bovine, Canine, Human, Marsupial, Mouse, Rat, Rhesus Monkey
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid