Invitrogen™ WNT3A Polyclonal Antibody, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ 500P251100UG

Catalog No. 500P251100

Description

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human Wnt-3a. Anti-Human Wnt-3a-specific antibody was purified by affinity chromatography employing an immobilized Human Wnt-3a matrix. Sandwich ELISA: To detect Human Wnt-3a by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human Wnt-3a (500-P251Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Wnt-3a. Western Blot: To detect Human Wnt-3a by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human Wnt-3a is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

Wnt3a, part of the WNT gene family, consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. Study of its expression in the teratocarcinoma cell line NT2 suggests that it may be implicated in the early process of neuronal differentiation of NT2 cells induced by retinoic acid. This gene is clustered with WNT3, another family member, in the chromosome 17q21 region.

Specifications

• Antigen: WNT3A
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: WNT3A
• Gene Alias: MGC119418; MGC119419; MGC119420; Protein Wnt-3a; vestigial tail; vt; wingless-related MMTV integration site 3A; wingless-type MMTV integration site family member 3A; wingless-type MMTV integration site family, member 3 like; wingless-type MMTV integration site family, member 3A; Wnt 3a; Wnt family member 3A; wnt[a]; wnt3; wnt3 l; wnt3 like; Wnt3a; Wnt-3a; WNT3A protein; wnt3l; Zwnt[a]
• Host Species: Rabbit
• Purification Method: Antigen Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 89780
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: ELISA, Western Blot
• Concentration: 0.1-1.0 mg/mL
• Formulation: PBS with no preservative
• Gene Accession No.: P56704
• Gene Symbols: WNT3A
• Immunogen: E.coli-derived Recombinant Human Wnt-3a.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody