Invitrogen™ WNT7A/WNT7B Polyclonal Antibody

Catalog No.	Quantity
PIPA547430	100 μg

Catalog No. PIPA547430 Supplier Invitrogen™ Supplier No. PA547430

Description

Goat Polyclonal Antibody

In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant mouse (rm) Wnt-1, rmWnt-3a, rmWnt-4, rmWnt-5a, rmWnt-5b, rmWnt-9a, rmWnt-11, and rmWnt-16 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.

Wnt proteins constitute a family of secreted glycoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

Specifications

• Antigen: WNT7A/WNT7B
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Classification: Polyclonal
• Concentration: 0.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 5% trehalose and No Preservative
• Gene: WNT7A
• Gene Accession No.: O00755, P56706
• Gene Alias: AI849442; postaxial hemimelia; protein Wnt-7a; Protein Wnt-7b; proto-oncogene Wnt7a protein; px; tw; wingless-related MMTV integration site 7A; wingless-related MMTV integration site 7B; wingless-type MMTV integration site 7A; wingless-type MMTV integration site family member 7A; wingless-type MMTV integration site family member 7B; wingless-type MMTV integration site family, member 7A; wingless-type MMTV integration site family, member 7B; Wnt family member 7A; Wnt family member 7B; Wnt7a; Wnt-7a; Wnt7b; Wnt-7b
• Gene Symbols: WNT7A, WNT7B
• Host Species: Goat
• Immunogen: E. coli-derived recombinant human Wnt-7a Leu32-Glu72, Thr216-Arg292.
• Purification Method: Antigen Affinity Chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7476, 7477
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG