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WT1 Mouse anti-Human, Clone: 6F-H2, Abnova™

Catalog No. 89935109 Shop All Abnova Corporation Products
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89-935-109 100 μg
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Catalog No. 89-935-109 Supplier Abnova Corporation Supplier No. MAB13454
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Description

Mouse monoclonal antibody raised against partial recombinant human WT1.

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]

Specifications

Antigen WT1
Applications Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed)
Classification Monoclonal
Clone 6F-H2
Conjugate Unconjugated
Description Mouse monoclonal antibody raised against partial recombinant human WT1.
Dilution Flow Cytometry (0.5-1 ug/106 cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
Formulation In 1mM PBS.
Gene Alias GUD/WAGR/WIT-2/WT33
Gene Symbols WT1
Host Species Mouse
Immunogen Recombinant protein corresponding to amino acids 1-181 of human WT1.
Purification Method Protein A/G purification
Quantity 100 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 7490
Test Specificity The monoclonal antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids.
Target Species Human
Content And Storage Store at -20 to -80°C.
Aliquot to avoid repeated freezing and thawing.
Form Liquid
Isotype IgG1 κ
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