WT1 Mouse anti-Human, Clone: 6F-H2, Abnova™

Mouse monoclonal antibody raised against partial recombinant human WT1.

Supplier:  Abnova Corporation MAB13453

Catalog No. 89-935-108

Description

This gene encodes a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. Multiple transcript variants, resulting from alternative splicing at two coding exons, have been well characterized. There is also evidence for the use of non-AUG (CUG) translation initiation site upstream of, and in-frame with the first AUG, leading to additional isoforms. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq]

Specifications

• Antigen: WT1
• Classification: Monoclonal
• Conjugate: Unconjugated
• Dilution: Flow Cytometry (0.5-1 ug/10⁶ cells in 0.1 mL) Immunofluorescence (0.5-1 ug/mL) Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) (0.5-1 ug/mL) The optimal working dilution should be determined by the end user.
• Gene Alias: GUD/WAGR/WIT-2/WT33
• Host Species: Mouse
• Purification Method: Protein A/G purification
• Regulatory Status: RUO
• Gene ID (Entrez): 7490
• Target Species: Human
• Form: Liquid

• Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (PFA fixed)
• Clone: 6F-H2
• Description: Mouse monoclonal antibody raised against partial recombinant human WT1.
• Formulation: In 1mM PBS (0.05% BSA, 0.05% sodium azide).
• Gene Symbols: WT1
• Immunogen: Recombinant protein corresponding to amino acids 1-181 of human WT1.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Test Specificity: The monoclonal antibody reacts with all isoforms of the full-length WT1 and also identifies WT1 lacking exon 2-encoded amino acids.
• Content And Storage: Store at 4°C.
• Isotype: IgG1 κ