Invitrogen™ XBP1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA595260

Catalog No. PIPA595260

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: MCF-7 whole cell, MM231 whole cell, MM453 whole cell, SKOV whole cell, HELA whole cell. IHC: mouse lung tissue, rat lung tissue, human tonsil tissue. Flow: HepG2 cell. Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

Specifications

• Antigen: XBP1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: Xbp1
• Gene Alias: D11Ertd39e; Hepatocarcinogenesis-related transcription factor; HTF; OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB5; TREB-5; X-box binding protein 1; X-box-binding protein 1; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; XBP1; XBP-1; XBP2
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22433, 289754, 7494
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 5mg BSA and 0.05mg sodium azide
• Gene Accession No.: O35426, P17861, Q9R1S4
• Gene Symbols: Xbp1
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human XBP1 (59-85 aa RKRQRLTHLSPEEKALRRKLKNRVAAQ).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG