Invitrogen™ XBP1 Polyclonal Antibody

Catalog No.	Quantity
PIPA578232	100 μL

Catalog No. PIPA578232 Supplier Invitrogen™ Supplier No. PA578232

Description

Rabbit Polyclonal Antibody

Positive Control: HepG2, HepG2 (3μM Thapsigargin treatment for 12 hr) Predicted Reactivity: Mouse (80%), Rat (82%), Pig (88%), Rhesus Monkey (97%), Bovine (90%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

XBP1 encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5.

Specifications

• Antigen: XBP1
• Applications: Western Blot, Immunocytochemistry
• Classification: Polyclonal
• Concentration: 2.2 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 20% glycerol and 0.025% ProClin 300; pH 7
• Gene: Xbp1
• Gene Accession No.: P17861
• Gene Alias: D11Ertd39e; Hepatocarcinogenesis-related transcription factor; HTF; OTTHUMP00000028682; Tax-responsive element-binding protein 5; tax-responsive element-binding protein 5 homolog; TREB5; TREB-5; X-box binding protein 1; X-box-binding protein 1; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; XBP1; XBP-1; XBP2
• Gene Symbols: Xbp1
• Host Species: Rabbit
• Immunogen: Recombinant protein encompassing a sequence within the center region of Human XBP1. The exact sequence is proprietary.
• Purification Method: Antigen affinity chromatography
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 7494
• Target Species: Human
• Content And Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG