XRCC1 Polyclonal Antibody, Rockland™

Chicken Polyclonal Antibody

Supplier:  Rockland Immunochemicals 200901MG5

Catalog No. 50-199-1298

Description

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. This purified antibody is directed against human XRCC1. This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70 kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA ligase III-alpha.

Specifications

• Antigen: XRCC1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: XRCC1
• Gene Alias: DNA repair protein XRCC1; RCC; X-ray repair complementing defective repair in Chinese hamster cells 1; X-ray repair cross complementing 1; x-ray repair cross-complementing group 1 protein; X-ray repair cross-complementing protein 1; Xrcc1; Xrcc-1
• Host Species: Chicken
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody

• Applications: ELISA, Immunofluorescence, Western Blot
• Concentration: 0.93 mg/mL
• Formulation: 0.002M sodium phosphate with 0.15M NaCl and 0.01% sodium azide; pH 7
• Gene Accession No.: P18887, Q60596
• Gene Symbols: XRCC1
• Immunogen: Anti-XRCC1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a N-Terminal portion of human XRCC1 conjugated to Keyhole Limpet Hemocyanin (KLH).
• Regulatory Status: RUO
• Gene ID (Entrez): 22594, 7515
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid