Invitrogen™ YBX1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA580242

Catalog No. PIPA580242

Description

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 μg/mL. Positive Control - WB: human Hela whole cell, human MCF-7 whole cell, human Jurkat whole cell, human T47D whole cell, rat spleen tissue, mouse RAW2647 whole cell. IHC: mouse testis tissue, rat testis tissue, human lung cancer tissue IHC-F: human placenta tissue. ICC/IF: A431 cell. Flow: A431 cell.

YBX1 binds to splice sites in pre-mRNA and regulates splice site selection. This protein binds and stabilizes cytoplasmic mRNA and contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors. It binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as HLA class II genes. It regulates the transcription of numerous genes and promotes separation of DNA strands that contain mismatches or are modified by cisplatin. It has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA (in vitro), and it may play a role in DNA repair.

Specifications

• Antigen: YBX1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: YBX1
• Gene Alias: 1700102N10Rik; BP8; BP-8; Byb1; C79409; Cbfa; CBF-A; CCAAT-binding; CCAAT-binding transcription factor 1 subunit A; CCAAT-binding transcription factor I subunit A; CSDA2; CSDB; dbpB; DNA binding protein B; DNA-binding protein B; EF1A; EFI-A; enhancer factor 1 alpha; enhancer factor 1 subunit A; enhancer factor I subunit A; major histocompatibility complex, class II, Y box-binding protein I; MDR NF1; MDR-NF1; MGC104858; MGC110976; MGC117250; Msy1; Msy-1; mYB-1a; Nsep1; NSEP-1; nuclease sensitive element binding protein 1; nuclease-sensitive element-binding protein 1; Y box binding protein 1; Y box protein 1; Y box transcription factor; YB1; YB-1; Y-box binding protein 1; Y-box transcription factor; Y-box-binding protein 1; YBX1
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 22608, 4904, 500538
• Content And Storage: -20°C
• Form: Lyophilized

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Western Blot, Immunocytochemistry
• Concentration: 500 μg/mL
• Formulation: PBS with 4mg trehalose and no preservative
• Gene Accession No.: P62960, P62961, P67809
• Gene Symbols: YBX1
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human YB1 (293-320aa ENPKPQDGKETKAADPPAENSSAPEAEQ).
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov