Invitrogen™ YTHDF1 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA5110587

Catalog No. PIPA5110587

Description

Immunogen sequence: VPKAPVPQQA PSPQAAPQPQ QVAQPLPAQP PALAQPQYQS PQQPPQTRWV APRNRNAAFG QSGGAGSDSN SPGNVQPNSA PSVESHPVLE KLKAAHSYNP KEFEWNLKSG R.

Specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency (PubMed:24284625, PubMed:26046440, PubMed:26318451). M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability (PubMed:24284625). Acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation (PubMed:26046440). Required to facilitate learning and memory formation in the hippocampus by enhancing protein synthesis upon neuronal stimulation: in response to neuronal stimulation, binds to m6A-containing neuronal mRNAs, promoting their translation, thereby contributing to learning and memory. Acts as a regulator of axon guidance by binding to m6A-containing ROBO3 transcripts, thereby promoting their translation. Acts as a negative regulator of antigen cross-presentation in myeloid dendritic cells. Acts by binding and promoting translation of m6A-containing transcripts encoding proteins involved in lysosomal degradation and phagosome maturation, leading to increased antigen degradation in myeloid dendritic cells. In the context of tumorigenesis, negative regulation of antigen cross-presentation limits the anti-tumor response by reducing efficiency of tumor-antigen cross-presentation. [UniProt].

Specifications

• Antigen: YTHDF1
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: YTHDF1
• Gene Alias: 2210410K23Rik; 8030473O16; C20orf21; DACA-1; DACA-1 homolog; Dermatomyositis associated with cancer putative autoantigen 1; dermatomyositis associated with cancer putative autoantigen 1 homolog; fb49c02; wu:fb49c02; wu:fi19h06; wu:fj87f09; wu:fp06c11; YTH domain family 1; YTH domain family protein 1; YTH domain family, member 1; YTH domain-containing family protein 1; YTH N(6)-methyladenosine RNA binding protein 1; YTH N6-methyladenosine RNA binding protein 1; Ythdf1; zgc:55657
• Host Species: Rabbit
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 228994, 296467, 54915
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Form: Liquid

• Applications: ELISA, Immunohistochemistry (Paraffin), Western Blot
• Concentration: 0.68 mg/mL
• Formulation: PBS with 50% glycerol and 0.05% ProClin 300; pH 7.3
• Gene Accession No.: P59326, Q9BYJ9
• Gene Symbols: YTHDF1
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 280-390 of human YTHDF1 (NP_0602682).
• Quantity: 100 μL
• Primary or Secondary: Primary
• Target Species: Human, Mouse, Rat
• Product Type: Antibody
• Isotype: IgG