Invitrogen™ ZBTB4 Polyclonal Antibody

Rabbit Polyclonal Antibody

Supplier:  Invitrogen™ PA521084

Catalog No. PIPA521084

Description

A suggested positive control is SK-N-SH cell lysate. PA5-21084 can be used with blocking peptide PEP-1198.

The ZBTB family of proteins is comprised of diverse zinc finger proteins that also contain a BTB (BR-C, ttk and bab) domain. Similar to Kaiso, a zinc-finger containing protein that can bind methylated CpGs, ZBTB4 can also bind methylated DNA and repress transcription. ZBTB4 has been shown to associate with the Sin3/histone deacetylase co-repressor and repress expression of P21CIP1 as part of a heterodimeric complex with Miz1. In cultured cells, depletion of ZBTB4 promotes cell cycle arrest in response to p53 activation and suppresses apoptosis through regulation of P21CIP1, suggesting that ZBTB4 is a critical determinant of the cellular response to p53 activation. HIPK2, a kinase that is involved in cellular proliferation and survival, phosphorylates and down-regulates ZBTB4 under normal cell growth conditions; this degradation increases with DNA damage.

Specifications

• Antigen: ZBTB4
• Classification: Polyclonal
• Conjugate: Unconjugated
• Gene: ZBTB4
• Gene Alias: 2310026P19Rik; 9230111I22Rik; KAISO-L1; KAISO-like zinc finger protein 1; Kiaa1538; mKIAA1538; Zbtb4; zinc finger and BTB domain containing 4; zinc finger and BTB domain-containing protein 4; ZNF903
• Host Species: Rabbit
• Purification Method: Antigen affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 57659, 75580
• Content And Storage: 4°C
• Form: Liquid

• Applications: Immunohistochemistry, Western Blot
• Concentration: 1 mg/mL
• Formulation: PBS with 0.02% sodium azide
• Gene Accession No.: Q5F293, Q9P1Z0
• Gene Symbols: ZBTB4
• Immunogen: An 18 amino acid peptide near the carboxy terminus of human ZBTB4.
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human, Mouse
• Product Type: Antibody
• Isotype: IgG